New palaeoceanographic constraints on the Eocene-Oligocene Transition in the Pacific (abstract of paper presented at AGU Fall Meeting, San Francisco, 6-10 Dec 2002)

The Eocene-Oligocene (E/O) transition represents perhaps the most pivotal phase in the shift from Cenozoic greenhouse to icehouse and is marked by the most pronounced shift in the calcite compensation depth (CCD) over the last 100 Myr. Yet detailed palaeoceanographic records for these important events are rare because of the lack of well-dated, expanded deep-sea sedimentary sections containing well-preserved calcareous microfossils. Recently, during Ocean Drilling Program Leg 199, we recovered a series of high-quality E/O sections across a latitudinal and depth transect in the central tropical Pacific Ocean. These sections provide an excellent opportunity to improve our understanding of the palaeoceanographic chain of events that took place across this important interval in the region of the world where the CCD perturbation is believed to be most extreme and in the largest ocean basin. Here, we report new high-resolution records of bulk sediment d13C, d18O and percent carbonate from ODP Sites 1217 through 1220. Our results show the following: (i) Bulk records from the central tropical Pacific have the potential to provide a remarkably clean and detailed chemostratigraphy for the E/O transition. (ii) CCD deepening occurred remarkably rapidly (initial depression <50 ka) and, in the most expanded section, at the shallowest end of the transect (Site 1218), as a two-step shift. (iii) The form of this two-step shift is strikingly similar to the bulk d18O record on the build up to Oi-1. (iv) The intermediate plateau that occurs between the two steps in the d18O series fits very well with the main ~100-120 ky eccentricity cycles observed in multi-sensor track data and their amplitude modulation (plateau = one cycle). (v) The interval of maximum CCD as defined by high carbonate sediment content (≥60% CO3) at the deeper end of the transect (Site 1220) correlates with the onset of Oi-1 and lasts for ~250 ka. (vi) Hitherto unrecorded extreme perturbations to low d18O and d13C values occur in the uppermost Eocene at Site 1218. (vii) d18O and d13C records from this site show significantly more structure within Oi-1 than published records (characteristic features of obliquity control, with a small imprint of precession)

Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples

Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for species-specific identification of C. jejuni, C. coli, C. lari, C. upsaliensis and of a hipO gene nucleotide probe for detection of C. jejuni by colony-blot hybridization were determined by testing a total of 75 reference strains of Campylobacter spp. and related organisms. The rt-PCR method allowed species-specific detection of Campylobacter spp. in naturally infected pig faecal samples after an enrichment step, whereas the hybridization approach enhanced the specific isolation of C. jejuni (present in minority to C. coli) from pigs. Conclusions: The rt-PCR was specific for Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis and the colony-blot hybridization approach provided an effective tool for isolation of C. jejuni from pig faecal samples typically dominated by C. coli. Significance and impact of study: Species differentiation between thermophilic Campylobacter is difficult by phenotypic methods and the developed rt-PCR provides an easy and fast method for such differentiation. Detection of C. jejuni by colony hybridization may increase the isolation rate of this species from pig feces

Establishment of effective metamodels for seakeeping performance in multidisciplinary ship design optimation

Ship design is a complex multidisciplinary optimization process to determine configuration variables that satisfy a set of mission requirements. Unfortunately, high fidelity commercial software for the ship performance estimation such as Computational Fluid Dynamics (CFD) and Finite Element Analysis (FEA) are computationally expensive and time consuming to execute and deter the ship designer’s ability to explore larger range of optimization solutions. In this paper, the Latin Hypercube Design was used to select the sample data for covering the design space. A comprehensive seakeeping evaluation index, The percentage of downtime, a comprehensive seakeeping evaluation index, was also used to evaluate the seakeeping performance within the short-term and long-term wave distribution in the Multidisciplinary Design Optimization (MDO) process. The five motions of ship seakeeping performance contained roll, pitch, yaw, sway and heave. Particularly, a new effective approximation modelling technique—Single-Parameter Lagrangian support vector regression ?SPL-SVR? was investigated to construct ship seakeeping metamodels to facilitate the application of MDO. By considering the effects of two ship speeds, the established metamedels of ship seakeeping performance for the short-term percentage downtime are satisfactory for seakeeping predictions during the conceptual design stage; thus, the new approximation algorithm provides an optimal and cost-effective solution for constructing the metamodels using the MDO process